Ovarian carcinoma cells are effectively transfected by polyethylenimine (PEI) derivatives
نویسندگان
چکیده
منابع مشابه
comparison of two delivery systems efficiency by using polyethylenimine (pei) for plasmid hpv16e7 dna transfection into cos-7 cells
objective: dna vaccines have been widely used to develop immunity against various pathogens including parasites and viruses. the potential of dna vaccine to induce an effective immune response is related to the expression levels of the encoded protein in eukaryotic cells. therefore, optimization of plasmid dna delivery system is a major concern in protein expression in order to make an efficien...
متن کاملDifferential intracellular distribution of DNA complexed with polyethylenimine (PEI) and PEI-polyarginine PTD influences exogenous gene expression within live COS-7 cells
BACKGROUND Polyethylenimine (PEI) is one of the most efficient and versatile non-viral vectors available for gene delivery. Despite many advantages over viral vectors, PEI is still limited by lower transfection efficiency compared to its viral counterparts. Considerable investigation is devoted to the modification of PEI to incorporate virus-like properties to improve its efficacy, including th...
متن کاملSelf-assembly of ternary insulin-polyethylenimine (PEI)-DNA nanoparticles for enhanced gene delivery and expression in alveolar epithelial cells.
Enhancing gene delivery and expression in alveolar epithelial cells could offer the opportunity for the treatment of acquired and inherited lung diseases. Here, we show that particle adsorption of human insulin (INS) is capable of increasing plasmid DNA (pDNA) delivery from polyethylenimine (PEI) nanoparticles specifically in alveolar epithelial cells. INS receptors were predominantly detected ...
متن کاملUsing transient transfected Chinese hamster ovary (CHO) cells by pET28a-LIC-NTF4 in gene therapy
Introduction: Neurotrophins, as a family of proteins, are responsible for induction of the survival, development, and function of neurons. Also, neurotropic factors are growth factors like Neurotrophins that help neurons survive. Moreover, Neurotrophins differentiate between progenitor cells so that neurons are formed. Despite the fact that the majority of mammalian brain neurons are produced p...
متن کاملComparison between polyethylene glycol (PEG)- and polyethylenimine (PEI)-mediated transformation of Aspergillus nidulans Authors
Genetic transformation of many filamentous fungi is carried out by a protocol that utilizes polyethylene glycol (PEG) and calcium ion (Ca). This method has remained practically unchanged for more than 20 years, but the roles these molecules play are not definitively understood. To gain a better understanding, we have compared PEG transformation to a protocol using polyethylenimine (PEI) that is...
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ژورنال
عنوان ژورنال: Cancer Gene Therapy
سال: 2000
ISSN: 0929-1903,1476-5500
DOI: 10.1038/sj.cgt.7700170